7. Conclusions

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(1) An axial scan of a homogeneous tissue by a confocal microscope yields experimental data that behaves as a single exponential: ρ exp(-μ zf).

(2) The values of ρ and μ uniquely map to a pair of optical property values, μs and g. Such confocal reflectance measurements allows separate meaurement of these two properties rather than measurement of the lumped parameter μs' = μs(1-g) as is obtained with optical measurements based on multiply scattered light in the diffusion regime (where photons move down concentration gradients).

(3) The variety of mouse tissues examined showed values of μs for the 488 nm wavelength of these experiments to be in the range of 300-600 cm-1. The values of g varied quite strongly (0.90-0.99) and demonstrated a strong effect on the confocal signal.

(4) This work is preliminary and there is still room for adjustments in the analysis.

(5) There is great opportunity in using anisotropy as a contrast agent since g is sensitive to the apparent particle size of scatterers that characterize the ultrastructure of a tissue (nuclei, mitochondria, collagen fibers, etc.).

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