@article{ramella03a,
  author = {Jessica C. Ramella-Roman and Paulo R. Bargo and Scott A. Prahl and Steven L. Jacques},
  title = {Evaluation of Spherical Particle Sizes with an Asymmetric Illumination Microscope},
  journal = {IEEE J. Selected Topics Quantum Electron.},
  year = {2003},
  volume = {9},
  pages = {301--306},
  abstract = {A polarized microscope system is used to perform goniometric measurements of light scattered by small particles. The light incident angle on a sample of monodispersed latex micro spheres is increased sequentially and a microscope objective lens collects scattered light from the samples. Light is only collected at angles greater than the objective lens numerical aperture, so that only light scattered by the spheres is collected. The experimental results were modeled with a Mie theory-based algorithm. Experiments conducted with micro spheres of diameter 1.03\,$\mu$m, 2.03\,$\mu$m and 6.4\,$\mu$m show that, by decreasing the objective lens numerical aperture from N.A.=0.55 to N.A.=0.0548, a more distinguishable scattering pattern is detectable. From these highly shaped curves, we found that the size of a sphere of nominal diameter 2.03\,$\mu$m was $2.11 \pm 0.06 \mu$m and a $6.4\,\mu$m sphere was $6.34 \pm 0.07 \mu$m.},
}