@article{prahl12a, author = {Scott A. Prahl and Rafael {L{\'o}pez P{\'a}ez} and Erik J. S{\'a}nchez and Kyle Juedes and Donald D. Duncan}, title = {Quantitative Retrieval Of Phase Information Using Differential Interference Contrast Microscopy}, journal = {Proceedings of the Oregon Academy of Science}, volume = {71}, pages = {}, year = {2012 abstract only}, abstract = {Differential interference contrast (DIC) microscopy uses a Nomarski prism to split the illumination light into two orthogonally polarized beams that are displaced (sheared) relative to one other. A second Nomarski prism recombines the two beams after the light passes through the objective. The DIC image is directly related to the spatial derivative of the phase of light passing through image. The derivative of the phase can be quantitatively recovered from a series of phase-stepped DIC images [1]. In this talk, we extend the process to recover the original phase of the object. By using derivative images in two orthogonal shear directions, the Fried technique enables reconstruction of the phase over a small 32x32 pixel tile. Stitching together multiple tiles allows the phase to be recovered over the entire image. We demonstrate the method by processing DIC images of a sample with known phase properties. [1] Duncan et al., JOSA:A, 28, 1297-1306 (2011).}, }