@article{buckley94a,
  author = {L. A. Buckley and S. L. Jacques and S. A. Prahl and K. W. Gregory},
  title = {Measurement of Serum Psoralen Levels Using Fluorescence},
  journal = {Lasers Surg. Med.},
  volume = {S6},
  pages = {1},
  year = {1994 abstract only},
  abstract = {Fluorescence detection of 8-methoxypsoralen (8-MOP) in blood serum may be a feasible method for determining drug levels on a real time basis. 8-MOP was delivered enterally to a 27\,kg pig at a dose of 11\,mg/kg (60\,mL of 5\,mg/mL 8-MOP in propylene glycol.) Blood samples were drawn at 0, 15, 30, 60, 90, and 120 min after administering 8-MOP and serum was prepared by centrifugation. Serum was diluted 1:2 in water and measured in a standard fluorimeter. Calibration of the fluorescence response of 8-MOP from 0.0 to 1.2\,mg/mL was linear over the range of serum measurements. The excitation wavelength was 365\,nm and peak emission was at approximately 450\,nm. Thin pathlength cuvettes (2\,mm excitation pathlength, 10\,mm emission pathlength) were used to ensure that all fluorescence was collected despite variation in penetration of excitation light into the sera. Using peak fluorescence as an assay, the 8-MOP serum concentration rose within 15 min to maximum levels and persisted through 120 min. The fluorescence detection method is sensitive enough to distinguish between slight variations in 8-MOP levels and thus would be a valuable diagnostic tool for matching light and drug levels to achieve therapeutic PUVA treatment or identifying sub-therapeutic drug levels.},
}