@inproceedings{atencio11, author = {J. A. {Delgado Atencio} and S. A. Prahl and S. {V{\'a}zquez y Montiel} and M. {Cunill Rodr{\'i}guez} and F. {Gutierrez Delgado} and J. {Castro Ramos}}, title = {Theoretical analysis and experimental validation of a two-fiber probe for biomedical spectroscopy applications}, booktitle = {International Commission for Optics 22 General Congress}, year = {2011, poster}, abstract = {The goal of the present work is to characterize theoretically and experimentally a simple two-fiber optic probe for spectroscopy applications in turbid biological media such as human skin. First, we perform a review of the wavelength dependence of optical parameters of this tissue. The validity of the diffusion approximation is evaluated for a simple model of skin in term of the restriction that scattering should dominate over absorption. The spectral bands where the diffusion theory fails are found for this model. Second, radially-resolved diffuse reflectance is compared using both Monte Carlo and the diffusion techniques. The optimal source-detector distance that minimizes errors is identified. In addition, a two-fiber optic probe was built and characterized to record spectra from in-vivo skin and phantoms mimicking some interesting real situation and in-vivo spectra from volunteers are used to evaluate an algorithm to extract intrinsic tissue optical properties. The derived optical properties are presented.}, }