POL: P&P Abstract VOL 66:PG 204

Potential Involvement of Both Type I and Type II Mechanisms in M13 Virus Inactivation by Methylene Blue Photosensitization

H. Abe*¹, K. Ikebuchi¹, S. J. Wagner², M. Kuwabara3, N. Kamo4 and S. Sekiguchi¹

¹Hokkaido Red Cross Blood Center, Sapporo, Japan
²Product Development Laboratory, The Jerome H. Holland Laboratory, American Red Cross Blood Services, Rockville, MD, USA;
3Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
4Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan

*To whom correspondence should be addressed at: Hokkaido Red Cross Blood Center, Yamanote 2-2, Nishi-ku, Sapporo 063, Japan. Fax: +81-11-613-4131.


We have investigated the mechanism of virus photoinactivation with methylene blue (MB) by conducting deuterium oxide (D2O), azide ion (N3-) and oxygen-dependent studies. Inactivation of M13 bacteriophage and singlet oxygen (¹O2) generation by MB photosensitization were irradiation dose dependent. Inactivation of M13 was enhanced by D2O and inhibited by N3-, suggesting that ¹O2 participates in M13 inactivation by MB photosensitization. However, N3- did not inhibit M13 inactivation completely. On the other hand, deoxygenating the reaction solution still caused 52-67% of M13 inactivation observed in the presence of oxygen. These results suggest that ¹O2-mediated (Type II) and sensitizer-mediated (Type I) reactions may both play roles in M13 inactivation by MB photosensitization.

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