Received 26 April 1996; accepted 25 September 1996
Concentration effects of methylene blue (MB) and oxygen on the photoinactivation rate of Qß bacteriophage were examined. The effect of initial virus concentration was verified on the similar f2 phage. The inactivation rate, k, is an increasing function of MB and O2 concentration and shows saturation with respect to MB concentration. Thus the results suggest that MB must adsorb to Qß sites and oxygen must be present for photoinactivation to occur. The inactivation rate is independent of the initial number of phage particles present before inactivation, indicating that inactivation does not depend upon interaction among viral particles or on surface effects. The results indicate that at least two different viral phenotypes exist within the wild-type Qß and f2 populations: one susceptible and the other resistant.
|January 1997 Table of Contents
|Photochemistry & Photobiology