These values for the molar extinction coefficient e in [cm-1/(moles/liter)] were compiled by using data from
To convert this data to absorbance A, multiply by the molar concentration and the pathlength. For example, if x is the number of grams per liter and a 1 cm cuvette is being used, then the absorbance is given by
(e) [(1/cm)/(moles/liter)] (x) [g/liter] (1) [cm]
A = ---------------------------------------------------
66,500 [g/mole]
using 66,500 as the gram molecular weight of hemoglobin.
To convert this data to absorption coefficient in (cm-1), multiply by the molar concentration and 2.303,
µa = (2.303) e (x g/liter)/(66,500 g Hb/mole)where x is the number of grams per liter. A typical value of x for whole blood is x=150 g Hb/liter.
lambda HbO2 Hb nm cm-1/M cm-1/M 450 68000 58000 460 45040 20600 480 27360 13360 500 20200 16360 507 19240 19240 510 19040 20000 520 23520 25080 522 25680 25680 540 57080 41120 542 57480 44000 549 49840 49840 555 36000 52160 560 33880 50160 569 45080 45080 577 61480 36800 579 54920 35440 586 28920 28920 600 3200 14600 605 1860 9496 615 1152 5776 625 732 4400 635 488 3796 645 396 3436 655 340 3244 665 292 3156 675 288 3028 685 272 2796 695 280 2424 705 300 1988 715 328 1628 725 368 1464 735 412 1464 745 480 1616 755 556 1756 765 616 1640 775 684 1340 785 736 1040 795 776 964 805 880 896 815 880 880 825 952 832 835 996 820 845 1048 820 855 1068 820 865 1116 820 875 1140 848 885 1168 832 895 1188 884 905 1208 896 915 1220 924 925 1228 860 935 1216 848 945 1212 756 955 1196 704 965 1176 616 975 1148 552 985 1108 424 995 1052 372