These values for the molar extinction coefficient e in [cm-1/(moles/liter)]
were compiled by using data
from
To convert this data to absorbance A, multiply by the molar concentration
and the pathlength. For example, if x is the number of grams per liter
and a 1 cm cuvette is being used, then the absorbance is given by
To convert this data to absorption coefficient in (cm-1), multiply by the
molar concentration and 2.303,
(e) [(1/cm)/(moles/liter)] (x) [g/liter] (1) [cm]
A = ---------------------------------------------------
66,500 [g/mole]
using 66,500 as the gram molecular weight of hemoglobin.
µa = (2.303) e (x g/liter)/(66,500 g Hb/mole)
where x is the number of grams per liter. A typical value of x for
whole blood is x=150 g Hb/liter.
lambda HbO2 Hb
nm cm-1/M cm-1/M
630 680 4280
640 440 3640
650 380 3420
660 320 3200
670 320 3080
680 320 2960
690 280 2560
700 320 2160
710 340 1840
720 360 1520
730 400 1500
740 440 1520
750 520 1620
760 600 1720
770 660 1420
780 720 1120
790 760 1020
800 800 920
810 860 880
820 920 840
830 980 840
840 1040 840
850 1060 800
860 1080 840
870 1120 840
880 1160 840
890 1180 860
900 1200 880
910 1220 920
920 1240 880
930 1240 800
940 1200 800
950 1200 720
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© SAP 4 March 1998