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This optical absorption measurement of Chlorophyll a were made by J. Li on 12-11-1997 using a Cary 3. The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min.
These measurements were scaled to make the molar extinction coefficient match the value of 111,700cm-1/M at 427.8nm (Strain, 1963).
The fluorescence emission spectrum of Chlorophyll a dissolved in diethyl ether. The excitation wavelength was 614nm. The quantum yield of this molecule is 0.32 (Weber, 1957). This spectrum was collected by on 12-11-1997 using a Spex FluoroMax. The excitation and emission monochromators were set at 1 mm, giving a spectral bandwidth of 4.25 nm. The data interval was 0.5 nm and the integration time was 2.0 sec.
Samples were prepared in 1cm pathlength quartz cells with absorbance less than 0.1 at the excitation and all emission wavelengths to uniformly illuminate across the sample, and to avoid the inner-filter effect. The dark counts were subtracted and the spectra were corrected for wavelength-dependent instrument sensitivity.
Dixon, J. M., M. Taniguchi and J. S. Lindsey (2005), "PhotochemCAD 2. A Refined Program with Accompanying Spectral Databases for Photochemical Calculations, Photochem. Photobiol., 81, 212-213.
Du, H., R.-C. A. Fuh, J. Li, L. A. Corkan and J. S. Lindsey (1998) PhotochemCAD: A computer-aided design and research tool in photochemistry. Photochem. Photobiol. 68, 141-142.
Seely, G. R. and J. S. Connolly (1986) Fluorescence of photosynthetic pigments in vitro. in Light emission by plants and bacteria. (Edited by Govindjee, J. Amesz and D. C. Fork), pp. 99-133, Academic Press.
Strain, H. H., M. R. Thomas and J. J. Katz (1963) Spectral absorption properties of ordinary and fully deuteriated chlorophylls a and b. Biochim. Biophys. Acta 75, 306-311.
Weber G. and F. W. J. Teale (1957) Determination of the absolute quantum yield of fluorescent solutions. Trans. Far. Trans. 53, 646-655.