trans-Stilbene This page summarizes the optical absorption and emission data of trans-Stilbene that is available in the PhotochemCAD package, version 2.1a (Du 1998, Dixon 2005). I reworked their data to produce these interactive graphs and to provide direct links to text files containing the raw and manipulated data. Although I have tried to be careful, I may have introduced some errors; the cautious user is advised to compare these results with the original sources.

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This optical absorption measurement of trans-Stilbene were made by J. Li on 12-11-1997 using a Cary 3. The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min.

These measurements were scaled to make the molar extinction coefficient match the value of 34,010cm-1/M at 294.0nm (Kvaran, 2000).

Original Data | Extinction Data


The fluorescence emission spectrum of trans-Stilbene dissolved in hexane. The excitation wavelength was 290nm. The quantum yield of this molecule is 0.044 (DiCesare, 2001). This spectrum was collected by on 12-11-1997 using a Spex FluoroMax. The excitation and emission monochromators were set at 1 mm, giving a spectral bandwidth of 4.25 nm. The data interval was 0.5 nm and the integration time was 2.0 sec.

Samples were prepared in 1cm pathlength quartz cells with absorbance less than 0.1 at the excitation and all emission wavelengths to uniformly illuminate across the sample, and to avoid the inner-filter effect. The dark counts were subtracted and the spectra were corrected for wavelength-dependent instrument sensitivity.

Original Data | Emission Data


Other extinction coefficient values include 29,000 cm-1/M (295.5 nm) in 95% ethanol (Beale, 1953). The fluorescence yield also has been reported to be 0.05 in methylcyclohexane/isohexane (2:1) (Allen, 1989) and 0.15 in glycerol (Gegiou, 1968). The solvent mixture used to determine the fluorescence yield (MCH/IH) presumably refers to methylcylcohexane and isohexane (Allen, 1989).


Allen, M. T. and D. G. Whitten (1989) The photophysics and photochemistry of a,w-diphenylpolyene singlet states. Chem. Rev. 89, 1691-1702.

Beale, R. N. and E. M. F. Roe (1953) Ultra-violet absorption spectra of trans- and cis-stilbenes and their derivatives. Part I. Trans- and cis-stilbenes. J. Chem. Soc. 2755-2763.

DiCesare N. and J. R. Lakowicz (2001) Spectral properties of fluorophores combining the boronic acid group with electron donor or withdrawing groups. Implication in the development of fluorescence probes for saccharides. J. Phys. Chem. A 105, 6834-6840.

Dixon, J. M., M. Taniguchi and J. S. Lindsey (2005), "PhotochemCAD 2. A Refined Program with Accompanying Spectral Databases for Photochemical Calculations, Photochem. Photobiol., 81, 212-213.

Du, H., R.-C. A. Fuh, J. Li, L. A. Corkan and J. S. Lindsey (1998) PhotochemCAD: A computer-aided design and research tool in photochemistry. Photochem. Photobiol. 68, 141-142.

Gegiou D., K. A. Muszkat and E. Fischer (1968) Temperature dependence of photoisomerization. VI. The viscosity effect. J. Am. Chem. Soc. 90, 12-18.

Kvaran, Á., Á. E. Konradsson, C. Evans and J. K. F. Geirsson (2000) -NMR and UV-vis spectroscopy of chlorine substituted stilbenes: conformational studies. J. Mol. Str. 553, 79-90.