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This optical absorption measurement of Thiacarbocyanine (C3) were made by J. Li on 12-11-1997 using a Cary 3. The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min.
These measurements were scaled to make the molar extinction coefficient match the value of 126,000cm-1/M at 559.0nm (Tredwell, 1979).
The fluorescence emission spectrum of Thiacarbocyanine (C3) dissolved in ethanol. The excitation wavelength was 545nm. The quantum yield of this molecule is 0.05 (Kuzmin, 1978). This spectrum was collected by on 06-25-1997 using a Spex FluoroMax. The excitation and emission monochromators were set at 1 mm, giving a spectral bandwidth of 4.25 nm. The data interval was 0.5 nm and the integration time was 2.0 sec.
Samples were prepared in 1cm pathlength quartz cells with absorbance less than 0.1 at the excitation and all emission wavelengths to uniformly illuminate across the sample, and to avoid the inner-filter effect. The dark counts were subtracted and the spectra were corrected for wavelength-dependent instrument sensitivity.
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Kuzmin, V. A. and A. P. Darmanyan (1978) Study of sterically hindered short-lived isomers of polymethine dyes by laser photolysis. Chem. Phys. Lett. 54, 159-163.
Rentsch, S.; R. Danielius and R. Gadonas (1984) Bestimmung von Lebensdauern und Transientenabsorptionsspektren von Polymethinfarbstoffen aus pikosekunden-spektroskopischen Messungen. J. Signalaufz.-Mater. 12, 319-328.
Tredwell, C. J. and C. M. Keary (1979) Picosecond time resolved fluorescence lifetimes of the polymethine and related dyes. Chem. Phys. 43, 307-316.