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This optical absorption measurement of Merocyanine 540 were made by R.-C. A. Fuh in the summer of 1995 using a Cary 3. The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min.
These measurements were scaled to make the molar extinction coefficient match the value of 138,000cm-1/M at 560.2nm (Dixit, 1983).
The fluorescence emission spectrum of Merocyanine 540 dissolved in ethanol. The excitation wavelength was 530nm. The quantum yield of this molecule is 0.39 (Hoebeke, 1990). This spectrum was collected by in the summer of 1995 using a Spex FluoroMax. The excitation and emission monochromators were set at 1 mm, giving a spectral bandwidth of 4.25 nm. The data interval was 0.5 nm and the integration time was 2.0 sec.
Samples were prepared in 1cm pathlength quartz cells with absorbance less than 0.1 at the excitation and all emission wavelengths to uniformly illuminate across the sample, and to avoid the inner-filter effect. The dark counts were subtracted and the spectra were corrected for wavelength-dependent instrument sensitivity.
Aramendia, P.F., M. Krieg, C. Nitsch, E. Bittersmann and S. E. Braslavsky (1988) The photophysics of merocyanine 540. A comparative study in ethanol and in liposomes. Photochem. Photobiol. 48, 187-194.
Benniston, A. C., A. Harriman and K. S. Gulliya (1994) Photophysical properties of merocyanine 540 derivatives. J. Chem. Soc. Faraday Trans. 90, 953-961.
Dixit, N. S. and R. A. Mackay (1983) Absoption and emission characteristics of Merocyanine 540 in microemulsions. J. Am. Chem. Soc. 105, 2928-2929.
Dixon, J. M., M. Taniguchi and J. S. Lindsey (2005), "PhotochemCAD 2. A Refined Program with Accompanying Spectral Databases for Photochemical Calculations, Photochem. Photobiol., 81, 212-213.
Du, H., R.-C. A. Fuh, J. Li, L. A. Corkan and J. S. Lindsey (1998) PhotochemCAD: A computer-aided design and research tool in photochemistry. Photochem. Photobiol. 68, 141-142.
Hoebeke, M., J. Piette, and A. van de Vorst (1990) Viscosity-dependent isomerization and fluorescence yields of merocyanine 540. J. Photochem. Photobiol. 4, 273-282.