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This optical absorption measurement of Vitamin B12 were made by R.-C. A. Fuh in the summer of 1995 using a Cary 3. The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min.
These measurements were scaled to make the molar extinction coefficient match the value of 27,500cm-1/M at 361.0nm (Hill, 1964).
Original Data | Extinction Data
Dixon, J. M., M. Taniguchi and J. S. Lindsey (2005), "PhotochemCAD 2. A Refined Program with Accompanying Spectral Databases for Photochemical Calculations, Photochem. Photobiol., 81, 212-213.
Du, H., R.-C. A. Fuh, J. Li, L. A. Corkan and J. S. Lindsey (1998) PhotochemCAD: A computer-aided design and research tool in photochemistry. Photochem. Photobiol. 68, 141-142.
Fugate, R. D., C.-A. Chin and P.-S. Song (1976) A spectroscopic analysis of vitamin B12 derivatives. Biochim. Biophys. Acta 421, 1-11.
Hill, J. A., J. M. Pratt and R. J. P. Williams (1964) The chemistry of vitamin B12. Part I. The valency and spectrum of the coenzyme. J. Chem. Soc. (London) 5149-5153.
Pratt, J. M. and R. G. Thorp (1966) The chemistry of vitamin B12. Part V. The class (b) character of the cobaltic ion. J. Chem. Soc. A, 187-191.