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This optical absorption measurement of Guanine were made by R.-C. A. Fuh on 06-24-1997 using a Cary 3. The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min.
These measurements were scaled to make the molar extinction coefficient match the value of 10,700cm-1/M at 243.0nm (Fasman, 1975).
Callis, P. R. (1979) Polarized fluorescence and estimated lifetimes of the DNA bases at room temperature. Chem. Phys. Lett. 61, 563-567.
Dixon, J. M., M. Taniguchi and J. S. Lindsey (2005), "PhotochemCAD 2. A Refined Program with Accompanying Spectral Databases for Photochemical Calculations, Photochem. Photobiol., 81, 212-213.
Du, H., R.-C. A. Fuh, J. Li, L. A. Corkan and J. S. Lindsey (1998) PhotochemCAD: A computer-aided design and research tool in photochemistry. Photochem. Photobiol. 68, 141-142.
Fasman, G. D., Editor (1975) Handbook of Biochemistry and Molecular Biology, 3rd Edition, Nucleic Acids, Volume I, pp. 65-215, CRC Press, Cleveland, Ohio.
Longworth, J. W., R. O. Rahn and R. G. Shulman (1966) Luminescence of pyrimidines, purines, nucleosides and nucleotides at 77 ÃÂÃÂ°K. The effect of ionization and tautomerization. J. Chem. Phys. 45, 2930-2939.