Nile Blue This page summarizes the optical absorption and emission data of Nile Blue that is available in the PhotochemCAD package, version 2.1a (Du 1998, Dixon 2005). I reworked their data to produce these interactive graphs and to provide direct links to text files containing the raw and manipulated data. Although I have tried to be careful, I may have introduced some errors; the cautious user is advised to compare these results with the original sources.

You can resize any of the graphs by clicking and dragging a rectangle. If you hover the mouse over the graph, you will see a pop-up showing the coordinates. One of the icons in the upper right corner will let you export the graph in other formats.


This optical absorption measurement of Nile Blue were made by R.-C. A. Fuh on 06-14-1995 using a Cary 3. The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min.

These measurements were scaled to make the molar extinction coefficient match the value of 76,800cm-1/M at 626.8nm (Birge, 1987).

Original Data | Extinction Data


The fluorescence emission spectrum of Nile Blue dissolved in methanol. The excitation wavelength was 540nm. The quantum yield of this molecule is 0.27 (Sens, 1981). This spectrum was collected by on 06-14-1995 using a .

Original Data | Emission Data


The fluorescence yield is 0.26 in ethylene glycol at 24 C, and 0.11 in ethanol at 61 C (Sens, 1981).


Birge, R. R. (1987) “Kodak Laser Dyes,” Kodak publication JJ-169.

Dixon, J. M., M. Taniguchi and J. S. Lindsey (2005), "PhotochemCAD 2. A Refined Program with Accompanying Spectral Databases for Photochemical Calculations, Photochem. Photobiol., 81, 212-213.

Du, H., R.-C. A. Fuh, J. Li, L. A. Corkan and J. S. Lindsey (1998) PhotochemCAD: A computer-aided design and research tool in photochemistry. Photochem. Photobiol. 68, 141-142.

Sens, R. and K. H. Drexhage (1981) Fluorescence quantum yield of oxazine and carbazine laser dyes. J. Luminesc. 24, 709-712.