These values for the molar extinction coefficient e in [cm-1/(moles/liter)] were compiled by Scott Prahl using data from
To convert this data to absorbance A, multiply by the molar concentration and the pathlength. For example, if x is the number of grams per liter and a 1 cm cuvette is being used, then the absorbance is given by
(e) [(1/cm)/(moles/liter)] (x) [g/liter] (1) [cm]
A = ---------------------------------------------------
64,500 [g/mole]
using 64,500 as the gram molecular weight of hemoglobin.
To convert this data to absorption coefficient in (cm-1), multiply by the molar concentration and 2.303,
µa = (2.303) e (x g/liter)/(64,500 g Hb/mole)
where x is the number of grams per liter. A typical value of x for whole blood is x=150 g Hb/liter.
lambda Hb02 Hb nm cm-1/M cm-1/M 250 106112 112736 252 105552 112736 254 107660 112736 256 109788 113824 258 112944 115040 260 116376 116296 262 120188 117564 264 124412 118876 266 128696 120208 268 133064 121544 270 136068 122880 272 137232 123096 274 138408 121952 276 137424 120808 278 135820 119840 280 131936 118872 282 127720 117628 284 122280 114820 286 116508 112008 288 108484 107140 290 104752 98364 292 98936 91636 294 88136 85820 296 79316 77100 298 70884 69444 300 65972 64440 302 63208 61300 304 61952 58828 306 62352 56908 308 62856 57620 310 63352 59156 312 65972 62248 314 69016 65344 316 72404 68312 318 75536 71208 320 78752 74508 322 82256 78284 324 85972 82060 326 89796 85592 328 93768 88516 330 97512 90856 332 100964 93192 334 103504 95532 336 104968 99792 338 106452 104476 340 107884 108472 342 109060 110996 344 110092 113524 346 109032 116052 348 107984 118752 350 106576 122092 352 105040 125436 354 103696 128776 356 101568 132120 358 97828 133632 360 94744 134940 362 92248 136044 364 89836 136972 366 88484 137900 368 87512 138856 370 88176 139968 372 91592 141084 374 95140 142196 376 98936 143312 378 103432 144424 380 109564 145232 382 116968 145232 384 125420 148668 386 135132 153908 388 148100 159544 390 167748 167780 392 189740 180004 394 212060 191540 396 231612 202124 398 248404 212712 400 266232 223296 402 284224 236188 404 308716 253368 406 354208 270548 408 422320 287356 410 466840 303956 412 500200 321344 414 524280 342596 416 521880 363848 418 515520 385680 420 480360 407560 422 431880 429880 424 376236 461200 426 326032 481840 428 283112 500840 430 246072 528600 432 214120 552160 434 165332 552160 436 132820 547040 438 119140 501560 440 102580 413280 442 92780 363240 444 81444 282724 446 76324 237224 448 67044 173320 450 62816 103292 452 58864 62640 454 53552 36170 456 49496 30698.8 458 47496 25886.4 460 44480 23388.8 462 41320 20891.2 464 39807.2 19260.8 466 37073.2 18142.4 468 34870.8 17025.6 470 33209.2 16156.4 472 31620 15310 474 30113.6 15048.4 476 28850.8 14792.8 478 27718 14657.2 480 26629.2 14550 482 25701.6 14881.2 484 25180.4 15212.4 486 24669.6 15543.6 488 24174.8 15898 490 23684.4 16684 492 23086.8 17469.6 494 22457.6 18255.6 496 21850.4 19041.2 498 21260 19891.2 500 20932.8 20862 502 20596.4 21832.8 504 20418 22803.6 506 19946 23774.4 508 19996 24745.2 510 20035.2 25773.6 512 20150.4 26936.8 514 20429.2 28100 516 21001.6 29263.2 518 22509.6 30426.4 520 24202.4 31589.6 522 26450.4 32851.2 524 29269.2 34397.6 526 32496.4 35944 528 35990 37490 530 39956.8 39036.4 532 43876 40584 534 46924 42088 536 49752 43592 538 51712 45092 540 53236 46592 542 53292 48148 544 52096 49708 546 49868 51268 548 46660 52496 550 43016 53412 552 39675.2 54080 554 36815.2 54520 556 34476.8 54540 558 33456 54164 560 32613.2 53788 562 32620 52276 564 33915.6 50572 566 36495.2 48828 568 40172 46948 570 44496 45072 572 49172 43340 574 53308 41716 576 55540 40092 578 54728 38467.6 580 50104 37020 582 43304 35676.4 584 34639.6 34332.8 586 26600.4 32851.6 588 19763.2 31075.2 590 14400.8 28324.4 592 10468.4 25470 594 7678.8 22574.8 596 5683.6 19800 598 4504.4 17058.4 600 3200 14677.2 602 2664 13622.4 604 2128 12567.6 606 1789.2 11513.2 608 1647.6 10477.6 610 1506 9443.6 612 1364.4 8591.2 614 1222.8 7762 616 1110 7344.8 618 1026 6927.2 620 942 6509.6 622 858 6193.2 624 774 5906.8 626 707.6 5620 628 658.8 5366.8 630 610 5148.8 632 561.2 4930.8 634 512.4 4730.8 636 478.8 4602.4 638 460.4 4473.6 640 442 4345.2 642 423.6 4216.8 644 405.2 4088.4 646 390.4 3965.08 648 379.2 3857.6 650 368 3750.12 652 356.8 3642.64 654 345.6 3535.16 656 335.2 3427.68 658 325.6 3320.2 660 319.6 3226.56 662 314 3140.28 664 308.4 3053.96 666 302.8 2967.68 668 298 2881.4 670 294 2795.12 672 290 2708.84 674 285.6 2627.64 676 282 2554.4 678 279.2 2481.16 680 277.6 2407.92 682 276 2334.68 684 274.4 2261.48 686 272.8 2188.24 688 274.4 2115 690 276 2051.96 692 277.6 2000.48 694 279.2 1949.04 696 282 1897.56 698 286 1846.08 700 290 1794.28 702 294 1741 704 298 1687.76 706 302.8 1634.48 708 308.4 1583.52 710 314 1540.48 712 319.6 1497.4 714 325.2 1454.36 716 332 1411.32 718 340 1368.28 720 348 1325.88 722 356 1285.16 724 364 1244.44 726 372.4 1203.68 728 381.2 1152.8 730 390 1102.2 732 398.8 1102.2 734 407.6 1102.2 736 418.8 1101.76 738 432.4 1100.48 740 446 1115.88 742 459.6 1161.64 744 473.2 1207.4 746 487.6 1266.04 748 502.8 1333.24 750 518 1405.24 752 533.2 1515.32 754 548.4 1541.76 756 562 1560.48 758 574 1560.48 760 586 1548.52 762 598 1508.44 764 610 1459.56 766 622.8 1410.52 768 636.4 1361.32 770 650 1311.88 772 663.6 1262.44 774 677.2 1213 776 689.2 1163.56 778 699.6 1114.8 780 710 1075.44 782 720.4 1036.08 784 730.8 996.72 786 740 957.36 788 748 921.8 790 756 890.8 792 764 859.8 794 772 828.8 796 786.4 802.96 798 807.2 782.36 800 816 761.72 802 828 743.84 804 836 737.08 806 844 730.28 808 856 723.52 810 864 717.08 812 872 711.84 814 880 706.6 816 887.2 701.32 818 901.6 696.08 820 916 693.76 822 930.4 693.6 824 944.8 693.48 826 956.4 693.32 828 965.2 693.2 830 974 693.04 832 982.8 692.92 834 991.6 692.76 836 1001.2 692.64 838 1011.6 692.48 840 1022 692.36 842 1032.4 692.2 844 1042.8 691.96 846 1050 691.76 848 1054 691.52 850 1058 691.32 852 1062 691.08 854 1066 690.88 856 1072.8 690.64 858 1082.4 692.44 860 1092 694.32 862 1101.6 696.2 864 1111.2 698.04 866 1118.4 699.92 868 1123.2 701.8 870 1128 705.84 872 1132.8 709.96 874 1137.6 714.08 876 1142.8 718.2 878 1148.4 722.32 880 1154 726.44 882 1159.6 729.84 884 1165.2 733.2 886 1170 736.6 888 1174 739.96 890 1178 743.6 892 1182 747.24 894 1186 750.88 896 1190 754.52 898 1194 758.16 900 1198 761.84 902 1202 765.04 904 1206 767.44 906 1209.2 769.8 908 1211.6 772.16 910 1214 774.56 912 1216.4 776.92 914 1218.8 778.4 916 1220.8 778.04 918 1222.4 777.72 920 1224 777.36 922 1225.6 777.04 924 1227.2 776.64 926 1226.8 772.36 928 1224.4 768.08 930 1222 763.84 932 1219.6 752.28 934 1217.2 737.56 936 1215.6 722.88 938 1214.8 708.16 940 1214 693.44 942 1213.2 678.72 944 1212.4 660.52 946 1210.4 641.08 948 1207.2 621.64 950 1204 602.24 952 1200.8 583.4 954 1197.6 568.92 956 1194 554.48 958 1190 540.04 960 1186 525.56 962 1182 511.12 964 1178 495.36 966 1173.2 473.32 968 1167.6 451.32 970 1162 429.32 972 1156.4 415.28 974 1150.8 402.28 976 1144 389.288 978 1136 374.944 980 1128 359.656 982 1120 344.372 984 1112 329.084 986 1102.4 313.796 988 1091.2 298.508 990 1080 283.22 992 1068.8 267.932 994 1057.6 252.648 996 1046.4 237.36 998 1035.2 222.072 1000 1024 206.784