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Chlorophyll b

The chemical structure of Chlorophyll b is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey. This excellent program allows rapid comparison of spectra and enables one to do a variety of photochemically relevant calculations. Chemical Structure

Absorption

Absorption Spectrum

This is a graph of the molar extinction coefficient of Chlorophyll b dissolved in diethyl ether. It was measured by Junzhong Li on 12/11/97 [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. Chlorophyll b has a molar extinction coefficient of 159,100 M-1cm-1 at 435.8 nm [L. P. Vernon and G. R. Seely, The chlorophylls, Academic Press, 1966].

For fluorescence studies of diverse photosynthetic pigments, see [G. R. Seely and J. S. Connolly, "Fluorescence of photosynthetic pigments in vitro," in Light emission by plants and bacteria, (J. Govindjee and D. C. Fork, eds.), 99-133, 1986].

Original Data | Extinction Data

Fluorescence

Emission Spectrum

This is the fluorescence emission spectrum of Chlorophyll b dissolved in diethyl ether. The spectrum was taken by Junzhong Li on 12/11/97 using an excitation wavelength of 435 nm [H. Du, R. A. Fuh, J. Li, A. Corkan, J. S. Lindsey, "PhotochemCAD: A computer-aided design and research tool in photochemistry," Photochemistry and Photobiology, 68, 141-142, 1998]. The quantum yield is 0.117 [G. Weber and F. W. J. Teale, "Determination of the absolute quantum yield of fluorescent solutions," Trans. Far. Trans., 53, 646-655, 1957].

Original Data

This page and graphs were created by Scott Prahl.