This page summarizes the optical absorption and emission data of Chlorophyll b that is available in the PhotochemCAD package, version 2.1a (Du 1998, Dixon 2005). I reworked their data to produce these interactive graphs and to provide direct links to text files containing the raw and manipulated data. Although I have tried to be careful, I may have introduced some errors; the cautious user is advised to compare these results with the original sources.
This optical absorption measurement of Chlorophyll b were made by M. Taniguchi in 2001 using a Cary 3. The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min.
These measurements were scaled to make the molar extinction coefficient match the value of 159,100cm-1/M at 453.0nm (Vernon, 1966).
The fluorescence emission spectrum of Chlorophyll b dissolved in diethyl ether. The excitation wavelength was 435nm. The quantum yield of this molecule is 0.117 (Weber, 1957). This spectrum was collected by on 12-11-1997 using a Spex FluoroMax. The excitation and emission monochromators were set at 1 mm, giving a spectral bandwidth of 4.25 nm. The data interval was 0.5 nm and the integration time was 2.0 sec.
Samples were prepared in 1cm pathlength quartz cells with absorbance less than 0.1 at the excitation and all emission wavelengths to uniformly illuminate across the sample, and to avoid the inner-filter effect. The dark counts were subtracted and the spectra were corrected for wavelength-dependent instrument sensitivity.
Dixon, J. M., M. Taniguchi and J. S. Lindsey (2005), "PhotochemCAD 2. A Refined Program with Accompanying Spectral Databases for Photochemical Calculations, Photochem. Photobiol., 81, 212-213.
Du, H., R.-C. A. Fuh, J. Li, L. A. Corkan and J. S. Lindsey (1998) PhotochemCAD: A computer-aided design and research tool in photochemistry. Photochem. Photobiol. 68, 141-142.
Seely, G. R. and J. S. Connolly (1986) Fluorescence of photosynthetic pigments in vitro. in Light emission by plants and bacteria. (Edited by Govindjee, J. Amesz and D. C. Fork), pp. 99-133, Academic Press.
Vernon, L. P. and G. R. Seely (1966) The chlorophylls. Academic Press, NY.
Weber G. and F. W. J. Teale (1957) Determination of the absolute quantum yield of fluorescent solutions. Trans. Far. Trans. 53, 646-655.
© 5 March 2012 Scott Prahl