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Auramine O

Scott Prahl

Auramine O This page summarizes the optical absorption and emission data of Auramine O that is available in the PhotochemCAD package, version 2.1a (Du 1998, Dixon 2005). I reworked their data to produce these interactive graphs and to provide direct links to text files containing the raw and manipulated data. Although I have tried to be careful, I may have introduced some errors; the cautious user is advised to compare these results with the original sources.

The JavaScript libraries that I used to graph the data do not work with IE7 and earlier — you will not see the cool interactive graphs. Chrome seems to work best. You can resize any of the graphs by clicking and dragging a rectangle. If you hover the mouse over the graph, you will see a pop-up showing the coordinates. One of the icons in the upper right corner will let you export the graph in other formats.

Absorption

This optical absorption measurement of Auramine O were made by R.-C. A. Fuh on 06-12-1995 using a Cary 3. The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min.

These measurements were scaled to make the molar extinction coefficient match the value of 25,300cm-1/M at 431.2nm (Müller, 1975).

Original Data | Extinction Data

Fluorescence

The fluorescence emission spectrum of Auramine O dissolved in glycerol. The excitation wavelength was 400nm. The quantum yield of this molecule is 0.03 (Brey, 1977). This spectrum was collected by on 06-12-1995 using a Spex FluoroMax. The excitation and emission monochromators were set at 1 mm, giving a spectral bandwidth of 4.25 nm. The data interval was 0.5 nm and the integration time was 2.0 sec.

Samples were prepared in 1cm pathlength quartz cells with absorbance less than 0.1 at the excitation and all emission wavelengths to uniformly illuminate across the sample, and to avoid the inner-filter effect. The dark counts were subtracted and the spectra were corrected for wavelength-dependent instrument sensitivity.

Original Data | Emission Data

Notes

The fluorescence yield varies markedly with solvent viscosity, ranging from 0.0016 in methanol to 0.019 in n-decanol (Gautam, 1994).

References

Brey, L. A., G. B. Schuster and H. G. Drickamer (1977) High pressure studies of the effect of viscosity on fluorescence efficiency in crystal violet and auramine O. J. Chem. Phys. 67, 2648-2650.

Dixon, J. M., M. Taniguchi and J. S. Lindsey (2005), "PhotochemCAD 2. A Refined Program with Accompanying Spectral Databases for Photochemical Calculations, Photochem. Photobiol., 81, 212-213.

Du, H., R.-C. A. Fuh, J. Li, L. A. Corkan and J. S. Lindsey (1998) PhotochemCAD: A computer-aided design and research tool in photochemistry. Photochem. Photobiol. 68, 141-142.

Gautam, P. and A. Harriman (1994) Internal rotation in Auramine O. J. Chem. Soc. Faraday Trans. 90, 697-701.

Müller, W. and F. Gautier (1975) Interactions of heteroaromatic compounds with nucleic acids. A.T-specific non-intercalating DNA ligands. Eur. J. Biochem. 54, 385-394.


© 5 March 2012 Scott Prahl