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slide 12

A typical OCT image of a forearm shows two bright reflecting layers. The upper layer is due to scattering from the stratum corneum. Our direct comparison of the OCT images with the corresponding CLSM data, shows that the second bright reflecting band can be ascribed to scattering of light at fibrous structure in the dermis. Possibly, the signal is caused by the interface between the papillary and reticular dermis. Our analysis suggests that the marker measured with OCT, Doct, corresponds with UD obtained by CLSM. In other words, the second light-reflecting layer in OCT is due to scattering of light at collagen fibers in the dermis, of which the structure and location can also be observed with CLSM.


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