Confocal Microscopy

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Melanoma in skin of mouse

A sideview (x,z @ y) of mouse skin with a single larger melanoma lesion and multiple early lesions. The large melanoma is in the center at x = 100-200 um, z = 40-60 um, y = 60 um. Note the following features:

The mouse was a very black mouse whose skin strongly absorbed the blue laser light (488 nm wavelength) used in this confocal microscope. So only the upper 40 um of tissue are imaged, but the underlying dermis quickly runs out of light and cannot be imaged.

Occasionally, hair follicles will disrupt the surface, presenting as dark regions that disrupt the surface (eg., x=50, @y=145).